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The homogenate was centrifuged at 20000 g for 30 min at 4C

Isolation of glutathione conjugates, including a novel mixed glutathionylglucuronide conjugate, from the bile of rats treated intraperitoenally with pulegone led to the proposal that glutathione conjugation plays a major role in detoxication of the cytochrome P450-bioactivated pulegone metabolite (i.e

de Boer MGJ, Jolink H, Halkes CJM, van der Heiden PLJ, Kremer D, Falkenburg JHF, van de Vosse E, van Dissel JT: Influence of Polymorphisms in Innate Immunity Genes on Susceptibility to Invasive Aspergillosis after Stem Cell Transplantation.PLoS One2011, 6:e18403 100

This sudden hypotension triggers a baroreceptor reflex, which causes a compensatory reflex tachycardia

Scientific Reports , 7 , 39975