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However, a pNF can progress into an atypical neurofibromatous neoplasm of uncertain biological potential (ANNUBP) 9 typically as a result of a subsequent genetic mutation in CDKN2A 10,11 and, upon further genetic insult to additional tumour-suppressor genes, such as TP53 , PTEN or members of the PRC2 chromatin-remodelling complex, 12 an ANNUBP or a pNF can transform into a MPNST

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Additionally, performing control experiments with GST alone (without the fusion protein) can help identify and account for any non-specific binding