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To make any meaningful adjustments to the treatment plan and to survey findings, standard next steps are essential

Animal model SpragueDawley rats (68 weeks, 180230 g) obtained from Guangdong Vital River Laboratory Animal Technology Co., Ltd

( a ) Antibacterial assays show no significant growth inhibition of Bacillus subtilis 168, Staphylococcus aureus ATCC43300 or Shigella flexneri BS103 at 100 M compound concentration compared to 1% DMSO control after 24 h incubation at 37 C compared to antibiotic positive controls of ciprofloxacin and rifampicin

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Due to the distinct position of the conjugated N-terminal lipid between Cagri-CTR and Cagri-AMY 1 R, we also determined a structure of CTR-Gs in complex with an analogue of Cagri that lacked the lipid modification (CagriBB) to interrogate the contribution of the acylation to the conformation of Cagri-CTR complex