glutathione protandim parkinson& 39 Targeting the Cysteine Redox Proteome in Parkinson's Disease: The Role of Precursors and Beyond Microglial Activation and Antioxidant Responses

Membranes were incubated with primary antibodies diluted in 5% milk 1X TBST overnight at 4C: anti-Actin (1:3000) (Sigma, A2066), anti-NRF2 (1:1000) (Cell Signaling Technology #12721) and anti-GSR (1:1000) (ThermoFisher, PA5-29945), followed by washing and incubation with HRP-conjugated anti-rabbit IgG (1:2000) (CST#7074) in 5% milk 1X TBST (22C, 1 h)

The HPLC analysis measures the exact percentage of active target molecules

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Yamaguchi H, Wyckoff J, Condeelis J (2005) Cell migration in tumors

Moreover, it can also be used in combination with vincristine and etoposide (145)