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Identification of an atypical Grb2 carboxyl-terminal SH3 domain binding site in Gab docking proteins reveals Grb2-dependent and -independent recruitment of Gab1 to receptor tyrosine kinases

Background and isolation BPC-157 is a synthetic 15-amino-acid peptide with the sequence Gly-Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val (GEPPPGKPADDAGLV) and a monoisotopic molecular weight of approximately 1419.55 Da (Sikiric et al., 2018)

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Conversely D-NIe-Met-Ile- NH-(CH 2 )s-CONH 2 , which has low affinity for HGF (Figure 23) and exhibits little anti- HGF/Met activity, is unable to block dimerization at the concentration tested

Technical datasheet Identity Physicochemical Handling & storage Compliance What's Included Research-grade vial Lyophilised powder, sealed under inert gas Certificate of Analysis Batch-specific HPLC & MS verification Tamper-evident seal Intact seal confirms product integrity Discreet packaging Plain, unbranded outer packaging COA & Testing Every batch of BPC-157 is independently analysed by HPLC and Mass Spectrometry before dispatch