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We next asked the question whether the regulation of the ISR by cystine is mTORC1dependent

Computationally, we removed this linker and manually reorient Grx1 so that GSSG binding site was close and aligned with the two reactive cysteines of roGFP2

Stigmasterol alleviates interleukin-1beta-induced chondrocyte injury by down-regulatingsterol regulatory element binding transcription factor 2 to regulateferroptosis

Cells were harvested and resuspended in the same lysis buffer as for ecGFP, and lysed by passing through a Constant cell disruptor three times at 25, 28, and 30 kpsi

The V max of rHrGSTm1 was 3.367 0.81 mol/min/mg, and the K m was 2.208 0.76 M (Fig