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On the other hand, Joris et al

The reaction mixture containing the homogenate and DCFH-DA (5mmol/L) was incubated for 15min at room temperature

Plates were then centrifuged at 800 g for 30 min, and the supernatant transferred into new plates

Their stability was further evaluated via classical MD

Molecular hydrogen is the smallest molecule in existence, allowing it to rapidly diffuse through cell membranes, penetrate the blood-brain barrier, and access intracellular compartments like mitochondria