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The membranes were subsequently incubated with horseradish peroxidase-conjugated secondary antibodies (1:5000, Proteintech, SA00001-2, Chicago, USA) at room temperature for 1 h, and the signals were visualised using an enhanced chemiluminescence detection reagent (Sigma-Aldrich, USA)

(MOV 9317 kb) Microglia form a continuous phagocytotic barrier along the glial limitans by assuming a jellyfish morphology (download MOV The representative 15 hr time lapse was captured in a CX3CR1 mouse beginning 5 min post-compression injury

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