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Future work should pair super-resolution and functional imaging with organelle-addressed reporters, localized perturbations (isoform-specific NOX/NOS, ER oxidoreductases, RET modulators) and computational models that capture vectorial flow rather than free diffusion

In addition, the day before running the assay, the Seahorse XF Sensor Cartridge was hydrated with sterile water followed by the calibration step (200 L per well of Seahorse XF Calibrant Solution) the day of the experiment in a non-CO 2 37 C incubator

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In milk, heat-induced folate decrease amounted to 810%, 420%, and 4245% during pasteurization, ultra-heat treatment, and sterilization, resp

The expression of rHrGSTm1 was the highest in the supernatant of bacteria induced by 0.5 mM IPTG for 8 h at 37 C, and a single band of rHrGSTm1 was obtained by elution with 500 mm imidazole (Fig