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Single bacterial colonies were inoculated into 5 ml of LBampicillin medium and grown overnight at 37 C with 250 rpm shaking (Minitron, INFORS)

Intrathecal injection of P2X4R antisense oligonucleotides significantly delays the onset of morphine tolerance ( In summary, P2XRs interact with signaling pathways such as AMPAR and TLR4, establishing a feedback network that leads to an imbalance between neuronal excitation and inhibition

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Saber AT, Jensen KA, Jacobsen NR, Birkedal R, Mikkelsen L, Mller P, et al