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Samples were then desalted again, and fractionated to 12 fractions using a 57-minute linear gradient from 3% to 32% acetonitrile (ACN) in 10 mM ammonium bicarbonate pH 8.0, at a flow rate of 0.25 mL/min, with an Agilent 1100 HPLC

Thus, while optimizing balance among pro-estrogenic influences is a central focus in approaching fibroids, it is also important to consider how progesterone and the function of its receptors may be contributing to their growth