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The dark-light transition experiments were carried out as described previously [23]

In this study, we reveal the inhibitory mechanism of Norclomipramine, a tricyclic antidepressant, which inhibits NEDD4-mediated ubiquitin chain elongation by binding to a hydrophobic pocket in the Ub exosite of the N-lobe

Reduced/denatured RNase A was diluted to 20 M (~50-fold dilution) and incubated with 1 M GPx7 or its mutant, and 10 M PDI in a buffer (50 mM Tris/HCl, pH 7.4, and 300 mM NaCl) at 30C, and reactions were initiated by adding 50 M of H 2 O 2

Hyams, C., Camberlein, E., Cohen, J