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Because pKa influences: Stability Reactivity Antioxidant capacity Glutathiones thiol group (from cysteine) is crucial for neutralizing free radicals

doi: 10.1016/j.foodchem.2023.135423 48

These include botany, morphology, microscopy, high-performance thin-layer chromatography (HPTLC), high-performance liquid chromatography (HPLC), liquid chromatography with mass spectrometry (LC-MS), inductively coupled plasma mass spectrometry (ICP-MS) for heavy metals testing, spectrophotometric methods (UV-Vis, FTIR), testing for microbial contamination, and other standard scientific methods