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This led on to methods that require alkylation of the reactive sulfur atom as the trapping reaction 28

The first incorporates GSH into the mitochondria by inorganic phosphate exchange and the second by exchange of 2-oxoglutarate (27, 28, 64) (Figures 2, 4)

By measuring the ratio of heavy isotope-labelled peptides in urine samples using liquid chromatography (LC)-MS in multiple reaction monitoring (MRM) mode, we observed significantly higher Ggt1 levels in the OVX rats than in the control rats (Figure 3C, peptide determination results are shown in Supplementary Tables S9)

In this approach, the gene coding for glutamate decarboxylase (GAD), which converts glutamate (the major excitatory neurotransmitter) into GABA (the major inhibitory neurotransmitter) is delivered to the subthalamic nucleus (STN)

Diabetic retinopathy Chronic hyperglycemia can damage blood vessels in the retina and cause a potentially sight-threatening condition called diabetic retinopathy (83)