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Remodelling of chromatin, residual histone alterations alongside DNA methylation reflect the key epigenetic shifts that occur at the sperm RNAs, and level of sperm seems to play an important role too (Reference Giacone, Cannarella and Mongio20)

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This timely recovery is essential for an in vitro infant gut model designed to test digested foods, as it allows experiments to be performed in buffer rather than culture media