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doi: 10.1093/ije/dyr036 29

Vital signs are recorded at the start of the infusion and assessed again before the patient is discharged

in its normally contracted state, the sphincter prevents reflux of acidic duodenal contents into the bile duct

GST-tagged proteins are purified from clarified bacterial lysates using immobilized glutathione, and are eluted under mild, non-denaturing conditions to preserve the function of the target protein