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Antigen retrieval was performed by high-pressure heat in citrate buffer for 5 minutes

Limit initial running sessions to 10 to 15 minutes and assess response over the following 24 to 48 hours

This interaction was specific, as the NINJ1 knockdown significantly reduced PLA signal and neither NINJ1 nor xCT antibody alone led to significant PLA signals (Fig

p16 expression is weak in healthy cells, but in senescent cells, p16 binds to and inhibits cell cycle protein-dependent kinase 4/6 (CDK4/6) activity, thereby promoting retinoblastoma (RB)-dependent cell cycle arrest [29,30,31]

The cell can then convert that combined form back into active glutathione and start over