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After removal of the medium, the cells were washed three times with PBS and incubated with DCFH-DA (10 M) (Nanjing Jiancheng, China) at 37 C for 30 min

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Short-term HL stress induces hydrogen peroxide, ABA and oxylipin signals with related gene expression changes To get insights into the triggers underlying gene expression changes during HL stress and R, we first traced the components responsible for initiating the HL-responsive signaling cascades in Arabidopsis leaves in comparison to GL