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Preferred methods for determining the KD values of mAbs can be found in Harlow, et aL, Antibodies: A Laboratory Manual, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y., 1988), Coligan et aL, eds., Current Protocols in Immunology, Greene Publishing Assoc, and Wiley Interscience, N.Y., 1992, 1993, and Muller, Meth Enzymol 1983, which references are entirely incorporated herein by reference

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11, e307 doi:10.1371/journal.pbio.0050307 Images courtesy of Monroe (Stages of Biofilm Development), Sapi et al

mustelae , which colonizes ferrets, Ni-deficiency leads to the expression of an alternative Fe-containing urease to replace Ni-urease (Stoof et al., 2008)