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Recordings were conducted during the first 5 min after establishment of the wholecell condition

see Background section and Example 1 discussion) of about 100-150 nM/10 5 WBC, or about 127.8 19.8 nM/10 5 WBC, or about 127.8 nM/10 5 WBC, as measured by the Tietze method, for example

MK-677 is a first-in-class ghrelin mimetic that increases both GH and IGF-1 levels without altering cortisol or testosterone, offering anabolic potential with minimal endocrine disruption. Murphy et al., Nature Reviews Drug Discovery How Does MK-677 Work

engineered cells may be allowed to grow for 1-2 days in an enriched media, and then are switched to a selective media

Respective simulations indicated that predictions based on a compartmental approach described the observed oral C/t with reasonable accuracy (Figure S4)