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The LC flow was set at 0.2 ml min 1 , and the gradient was as follows: 10% A held for 2 min, 35% A at 18 min, 70% A at 22 min, 90% A at 22.1 min and held for 2.9 min, 10 % A at 25.1 min and held for 5 min

Complete loss of GPX4 is incompatible with viability, and pharmacological or genetic GPX4 inhibition is widely exploited to induce ferroptosis in cells and tissues

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