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Parasite pellets were resuspended in buffer (3.5 mM MgCl 2 , 110 mM KCl, 40 mM NaCl, 20 mM HEPES, 6 mM EDTA, pH 7.4) with protease inhibitors (0.01 mg of leupeptin A, 0.001 mg of pepstatin A, 0.35 mg of PMSF) lysed by three freeze/thaw cycles (liquid nitrogen and 37C water bath), and protein content was determined using Bio-Rad DC Protein Assay

Clinical experience from thousands of patients in optimization medicine practices supports its use, but the formal evidence lags behind the clinical adoption

Failure to reset hypoxia/Warburg effect after viral clearance in COVID-19 survivors, could eventually evoke PASC with metabolic impairments including new onset T2DM, myocardial infarction, chronic fatigue syndrome (CFS), brain fog, and blood clotting issues 141

BPC-157 is generally well-tolerated with minimal side effects

The most commonly reported side effect in animal studies was mild local irritation at the injection site, which resolved without sequelae