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One important caveat: wound serum from chronic wounds (such as diabetic ulcers) contains enzymes that break down GHK-Cu, which may limit topical efficacy on infected or chronic wounds

In reality, the human body tightly regulates blood pH between 7.35 and 7.45, and dietary bicarbonate has negligible influence on this system

Science 300 , 342344 (2003)

Given that this approach uses a selective ion pair technique, not a full scan technique like others, it can improve sensitivity by up to two orders of magnitude and the linear response over a wide dynamic range up to five orders of magnitude compared to full scan technique (Lange et al., 2008)

Leaves were cut with scissors and the slices incubated overnight in the dark at 23 C in F-Pin solution (10 mM MES pH 5.8, 0.32% Gamborgs B5 basal salt powder with bioWORLD vitamins, 0.4 M sucrose) containing 0.5% (w/v) cellulase Onozuka R10 and macerozyme R10 (SERVA Electrophoresis GmbH, Heidelberg, Germany)