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Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm

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Hybridomas that produced antibodies that specifically reacted with the proteins were cloned by limiting dilutions and grown in serum-free medium (Thermo Fisher Scientific)

This study demonstrated for the first time that disturbed NAD+ metabolism accelerates follicular atresia and ovarian reserve depletion by disrupting the microenvironment of oocytesomatic cell interactions, ultimately leading to a reproductive ageing phenotype [82]

Chung WY