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However, studies on targeted segments of the population have shown that the upper limit of 15mol/L is far too high in well-nourished populations without obvious vitamin deficiency

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Studies using up to 96 IU daily showed safety profiles similar to placebo, but staying within recommended ranges minimizes risk

Particle titers of purified viruses were quantified by making three separate dilutions of each virus in 0.1% SDS (Thermo Fisher) and vortexing for 5 minutes, spinning down 13,000 x rpm, 5 minutes, and then taking the average of the absorbance readings on the Nanophotometer N50-GO (Implen) to quantify particles/mL as per (Maizel et al., 1968)

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