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The reaction mixture was then transferred into a round-bottomed flask, DMS (4.0 equiv, 300 L) and Et 3 N (4.2 L, 0.03 mmol) were added and the flask was sealed for further 40 min at room temperature

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Every batch of FOXO4-DRI 10mg is independently tested by a third-party analytical laboratory using HPLC for purity and mass spectrometry for identity confirmation