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Description

Addition of propolis to irinotecan therapy prolongs survival in ehrlich ascites tumor-bearing mice

10.3390/metabo6040035 13 AloneziS.TusiimireJ.WallaceJ.DuftonM

Pacana and Sanyal 2012)

Many toxic and harmful substances we are exposed to on a daily basis require considerable amounts of glutathione in order to be eliminated through the detoxification process

The eluted proteins were subjected to size-exclusion chromatography (SEC) with a Superose 6 Increase 10/300 GL column (Cytiva) in the final buffer containing 25 mM HEPES pH 7.5, 150 mM NaCl and 1 mM DTT (buffer A, for biochemical assays) or 5 mM DTT (buffer B, for cryo-EM)