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In these studies, most patients responded to at least one melanoma antigen and many to multiple epitopes, whereas no single peptide emerged as a dominant target across patients

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directional ligation will typically displayed when a digested PCR product having a 5' EcoR I-treated end and a 3' BamH I-is ligated into a cloning vector that has a multiple cloning site digested with EcoR I and BamH I

Assessment of Intracellular GTP Levels Using Genetically Encoded Fluorescent Sensors