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3T3-L1 pre-adipocytes cells were cultured with standard culture media (DMEM, 4.5 g/L glucose, L-glutamine, sodium pyruvate, 10% FBS, 1% antibiotic-antimycotic solution) and grown for 48 h before initiating differentiation using the manufacturer's suggested protocol and modified from previous published work

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Although the serum HDL-c levels decreased near the significant levels in both trials arms, no significant difference was observed between the groups

[1] Whilst B12 supplementation is generally well-tolerated, some individuals report experiencing joint pain or aching after commencing treatment

Treatment duration depends on the underlying condition and clinical response