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(2) Initially regarded only for its potential as a sleep-including factor, DSIP was soon suggested to induce other potential actions, such as mitigating pain, sleep cycle regulation, and mitigating withdrawal

For the immunoperoxidase staining, sections were incubated for 1 h in biotin-conjugated secondary antibody (1:1000, Jackson Laboratories) and next for 1 h with an avidin-biotin complex (1:500, Vector Labs)

doi: 10.1016/j.neuron.2016.06.033 22 YuYZengZXieDChenRShaYHuangSet al

Tseng Y, Lai C, Wang Y, Chen W, Kao C, Chen C (2020) Possible increased risk of colonic diverticular disease from alcohol intoxication or abuse

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