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A saline-based infusion delivers hydration and selected nutrients gradually

This action removes two fibrinopeptides, exposing knobs and holes, and leading to a remarkable self-assembly in which fibrin monomers polymerise to make staggered oligomers, which themselves lengthen into protofibrils that aggregate laterally to make fibres, finally branching to create a three-dimensional network which represents the clots

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Every IV insertion creates potential bacterial entry points, which is why we maintain strict sterile protocols

To quantify reporter expression mice were perfused with artificial cerebral spinal fluid, followed by perfusion with 15 ml of 4% formaldehyde solution