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The measurement of carnitine and acyl-carnitine: application to the investigation of patients with suspected inherited disorders of mitochondrial fatty acid oxidation

Samples were then precipitated with 1 mL absolute MeOH at 40 C for 2 h, centrifuged (16,000 g ), and the obtained supernatants were evaporated, and the residue was resuspended and sonicated in 100 L 50 mM HEPES buffer (pH 7.4)