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Vector construction and transfection PCR-amplified human GCLC was cloned into p3xFLAG-CMV-10 or pGEX-5X-1

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linked dihydroartemisinin to a multi-armed PEG, and this coupling increased the dihydroartemisinin loading capacity, enhanced the water solubility, and increased the half-life of the drug in blood circulation, resulting in better inhibition of tumor growth (Dai et al., 2014)