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Glutathione Peroxidase 1 Promotes the Survival of Spiral Ganglion Neurons After Peroxynitrite Damage To clarify the role of GPX1 in the process of SGN injury caused by peroxynitrite, we performed experiments by increasing or inhibiting the expression of GPX1 in SGNs with the GPX1 mimic ebselen or using the gpx1 / mouse model

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We then transfected the silenced cells and the control cells with plasmids for expression of the ARE-luc reporter gene and the ORF of KEAP1 (wt or point mutated in the identified sensor residues), which lacks its 3UTR sequence and is therefore resistant to the shRNA used (Fig

Nappo et al, 1999), methionine load-induced hyperhomocysteinemia favors the occurrence of vascular spasm and thrombosis, and that endothelial dysfunction can be prevented by an antioxidant treatment (Chambers et al, 1999a