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Research has showed GHK-CUs effects on gene expression, with studies showing tuning of over 4,000 genes involved in many cellular processes

Additionally, PF treatment elevated the expression of liver kinase B1 (LKB1) and AMP-activated protein kinase (AMPK), which promoted PF-enhanced autophagic activity

LeBlanc et al., 2013

Briefly, template cDNA (1 l), SYBR green (2x) mixes (5 l), forward and reverse primers (0.5 l each) and total reaction volume up to 10 l was made using nuclease-free water