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Antagonistic effects of Bacillus subtilis-derived Chitosan nanoparticles on growth performance, stress biomarkers, and histological alterations of cadmium-intoxicated nile tilapia fingerlings

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Remove all PBS and add a volume of the following buffer: 50 mM Na-phosphate, 300 mM NaCl, pH 8.0, 0.01 % Tween 20 detergent, 0.5% to 1% Triton-X 100 detergent + protease inhibitors of your choice (200 L of this buffer for a T25 flask or 300-500 L for T75 flask)

doi: 10.1371/journal.ppat.1009300 70 ZhangBXuSLiuMWeiYWangQShenWet al

Its unique Pico-cell delivery system ensures maximum absorption and efficacy