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Table 1: In Vitro Protocol - ERK Activation and ROS in Neuronal Cells This protocol is based on studies using rat cerebellar granule cells [2] [3]

Importantly, we found that phosphorylation of serine 727 residue of STAT3, p-STAT3-S727, increased in hyperaminoacidemia-treated cells, further elevating Vgf transcription in an mTOR-dependent manner