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However, extensive optimization of conditions is required, as the cell death rate in cells subjected to this method is high [254]

Mice were nebulized (Pari Trek S Portable 459 Compressor Nebulizer Aerosol System, 047F45-LCS, PARI, Starnberg, Germany) with RFP-LSC in a single dose of 10 9 particles per mouse or 0.83 10 9 RFP-Lipo per mouse or 1.05 10 9 RFP-HEK per mouse, where equal RFP amount in RFP-LSC, RFP-HEK and RFP-Lipo were used

While Figure 6 is Hofstee linear plot, the K m and (V max ) obtained from the plot were 7.58 M and 0.47 nmoles ml -1 min -1

Pantothenic acid Pantothenic acid is involved in the biosynthesis of coenzyme A, cholesterol, fatty acids, and acetylcholine (45)