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Methylcobalamin is the bioactive form of B12

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348 Studies have also shown that two types of harmful bacteria in the gut, Fusobacterium nucleatum and Bacteroides fragilis , promote the proliferation of UC intestinal inflammatory cells by regulating miR-574-5p and miR-149-3p, respectively, which disrupts the integrity of the intestinal epithelial barrier

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Enzyme-linked immunosorbent assay (ELISA) Equal amounts of kidneys from each group were homogenized in lysate, and inflammatory cytokines, includingTNF-, IL-1, IL-6 were measured in the lysed supernatant using ELISA kits according to the manufacturers instructions (Thermo Fisher Scientific, United States)