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When a stroke occurs, due to increased paracellular and transcellular permeability along with endothelial injury, chemicals and fluids extravasate from the damaged BBB into the brain parenchyma resulting in vasogenic edema (Heo et al., 2005

Ann Intern Med 158(1):19 Lavonas EJ, Drennan IR, Gabrielli A, Heffner AC, Hoyte CO, Orkin AM, Sawyer KN, Donnino MW (2015) Part 10: special circumstances of resuscitation: 2015 American Heart Association guidelines update for cardiopulmonary resuscitation and emergency cardiovascular care

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This prevents cellular damage (like hemolysis) and discomfort

The 20 L reaction mixture was prepared as HERA SYBR green PCR Master Mix (2X) (Willowfort, UK), 2 L of cDNA fragments, 1 L (10 pmol) of each primer and 6 L of nuclease-free water