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In fact, standard cell culture media do not recapitulate the complexity of a human tumour microenvironment and adaptations to better mimic the physiological situation are needed to better understand metabolic rewiring in cancer

[PubMed: 16841072] 54

CEP192 wild-type control clones were chosen from sorted GFP-positive cells which did not contain the required CEP192 mutation

Targeted inactivation of fh1 causes proliferative renal cyst development and activation of the hypoxia pathway

However, the finding that GSH levels oscillated over a 24-hour period does provide supportive evidence that regulation of GSH levels may be circadian driven