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The primer sequences for RT-qPCR were listed in Table S2

APAP-induced senescence is most prevalent among cells at the perinecrotic area, where NAPQI-cys levels are high

Quantitative PCR (qPCR) analysis was performed using the KAPA SYBR Fast kit (Sigma) on a Rotor Gene Real-Time Cycler (Corbett Research)

You might notice slight bruising, redness, or tenderness at the injection site basically what you'd expect from any needle stick

This inaction is truly disheartening because, in a March 2009 radio address, you committed to work vigorously for strong food safety measures, noting "no parent should have to worry that their child is going to get sick from their lunch," and that, "many of the laws and regulations governing food safety in America have not been updated since they were written in the time of Teddy Roosevelt." Less than two years later, on January 4, 2011, you signed into law the FDA Food Safety Modernization Act (FSMA) -- passed by Congress with bipartisan and industry support -- which promised real action to better protect the public from preventable foodborne illness