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Holman RR, Paul SK, Bethel MA, Matthews DR, Neil HA

These foundational steps are essential for achieving reliable research outcomes

In vivo SYTOX green staining After zebrafish were immersed by 400 M DCA ( n = 6, 1 larvae/well), 5 M SYTOX Green nucleic acid stain was directly added into 96-well plate for 30 min in a dark place

A vial that has lost potency due to poor storage will look identical to a fresh one, but the medication inside may be partially or completely inactive