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Disulfides were reduced with 4 mM dithiothreitol (DTT) at RT for 1 h and then alkylated with 10 mM indole-3-acetic acid (IAA) for 15 min in the dark

Subsequent research studies have confirmed that the levels of ROS in AD patients are increased, and in severe clinical cases, the protein folding function of the endoplasmic reticulum is impaired, and the clearance of damaged proteins mediated by proteases and autophagy is reduced, which promotes the accumulation of amyloid- (A ) and TAU proteins 37

Cell Physiol Biochem 29:543550