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The serum homocysteine levels were determined using the ELISA method by laboratory kit (Axis-Shield Diagnostics, Scotland, UK)

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These recommendations assume normal absorption and dietary intake from animal-based foods such as meat, fish, dairy products, and eggs

The primary downstream pathway involves JAK2 trans-phosphorylation and subsequent activation of STAT5a/b, which drives transcription of key targets including hepatic IGF-1, IGFALS, and feedback regulators CISH and SOCS2

The benefits are felt almost immediately and typically last 57 days