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First separation was carried out with 15 mM ammonium bicarbonate in 90% water and 10% acetonitrile as mobile phase A, and mobile phase B was 15mM ammonium bicarbonate in 95% acetonitrile and 5% water

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In future articles, I will discuss other important functions of vitamin B12 , causes of vitamin B12 deficiency, and treatments to get your levels optimized

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