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Immediately after isolation, PBMC were seeded on a 96-well microplate at a density of 10 5 cells per well and incubated for 69.5 h at 37C and 5% CO 2 with or without stimulation of concanavalin A (ConA, 2.5 g/ml

It then explores mainstream and emerging therapeutic modalities, including pharmacological, surgical, and microbiotabased interventions

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Inject slowly and steadily to minimize leakage and support accurate delivery

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