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Transfection of plasmid/siRNA was performed using Lipo3000 (Thermo Fisher) according to the manufacturers instructions

Studies on vascular injury demonstrate BPC-157 protects endothelium from damage, prevents thrombosis formation, and promotes vessel healing

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and, c) using the set of mutagenic oligonucleotides to generate a set of T cell receptor (TCR)-encoding variant nucleic acids encoding a range of amino acid variations at each amino acid codon that was mutagenized, thereby producing a library of nucleic acids encoding a plurality of variant T cell receptors (TCRs)

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