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Although L-carnitine fulfils some of these requirements, acetyl-L-carnitine fulfills these requirements and also has the additional capability to provide an acetyl-CoA that can be oxidized for energy, used as a precursor for acetylcholine, or incorporated into glutamate, glutamine, GABA, and into lipids for myelination and cell growth

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At 12 hpi, cells were lysed by resuspending in 3 mL of mild lysis buffer (10 mM Tris, pH 7.4, 150 mM NaCl, 2 mM MgCl 2 1 mM EDTA, 1 mM DDT and 0.5 % Igepal) and incubated on ice for 10 min, followed by vortexing for 30 s

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