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Blood serum was allowed to clot in the S-monovette (Sarstedt AG & Co, Nmbrecht, Germany) for 30 min at room temperature before centrifugation at 2000 g for 10 min

While the majority of in vitro studies have reported a decreased responsiveness of hyperplastic parathyroid cells to changes in [Ca2+e] in vivo studies have not always confirmed this

Science 88 , 432433 (1938)

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The consistency of this finding across different research groups, statistical methods, and patient populations makes it one of the most robust conclusions in the hair loss treatment literature